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Effect of overexpression of ARE2, UPC2-1, and ACC1 on cell growth and ergosterol production. (A) Cell growth curves and (B) ergosterol production of the strains S1, S1-ARE2, S1-AU, and S1-AUAC in 250 mL shake flasks with 50 mL of YPD medium containing 50 g/L glucose. Samples for ergosterol quantification was collected after 48 h cultivation.
