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. 2021 Jun 29;9:681666. doi: 10.3389/fbioe.2021.681666

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Copyright © 2021 Sun, Lian, Zhu, Jiang, Li, Xue, Wu, Yang and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of gal80Δ-GAL1 promoter constructs on growth and ergosterol production. (A) Cell growth curves of strain S1-G-AUAC and the control strain S1-G; time course of (B) ergosterol yield and (C) ergosterol content of strains S1-G and S1-G-AUAC. Samples were collected at 16, 32, 48, 72, and 96 h during the flask fermentations. (D) Comparison of high-performance liquid chromatographic (HPLC) profiles of strains S1 (parent strain), S1-AUAC (constitutive overexpression of ARE2, UPC2-1, and ACC1), and S1-G-AUAC (inducible overexpression of ARE2, UPC2-1, and ACC1). All the strains were cultivated in 250 mL shake flasks with 50 mL of YPD medium containing 50 g/L glucose.