Table 2.
T. cruzi epitopes recognized by CD8+ T cell from mice (not tested in Chagas disease patients).
| aEpitope | bID | cAntigen Name | dMouse Model | EMHC Restriction | Reference |
|---|---|---|---|---|---|
| VDYNFTIV | Pep 77.2#
(TSA-1515-522) |
Trans-sialidase, putative/ surface glycoprotein/ trypomastigote surface antigen-1 (TSA-1) |
CL57BL/6 | H-2Kb | (55, 56) |
| VNHDFTVV | PA14# | Putative trans-sialidase, Group II/surface protien-1/amastigote surface protein-1 (ASP-1) | CL57BL/6 | H-2Kb | (57) |
| KNYPFSSI NTLVFPLV DNRQYSFV VNHRFTLV EKEANALYLWV |
PA5 PA6 PA7 PA8# PA10 |
Putative trans-sialidase, Group II/surface protein-2/amastigote surface protein-2 (ASP-2) | CL57BL/6 | H-2Kb
H-2Kb H-2Kb H-2Kb H-2Kb/H-2Db |
(57) |
| LSHNFTLV ANYDFTLV ANYKFTLV ANYNFTLV LSHDFTVV LSHNLTLV LSHSFTLV VNYDFTLV ANYNFTLL LLRPTTLV VNYDFTIV VGRPTTVV LHKRFTLV AKYNFTLL ANYKFTLL ANYNLTLV ANHRFTLV |
TSKB4#
TSKB18 TSKB20# TSKB21# TSKB24 TSKB27 TSKB60 TSKB74 TSKB80 TSKB84 TSKB89# TSKB92 TSKB96 TSKB252 TSKB260 TSKB306 TSKB388 |
Trans-sialidase, putative | CL57BL/6 TSKB20, TSKB18, TSK20/18 Tg mice (58) |
H-2Kb | (33, 58–63) |
| EVYSLVFARL NVLLYNRPL WLTDNTHIV NTWRDEYLGV KVGSDVFAV RVFTSAVLL |
F-TS-2 NF-TS-2 NF-TS-3 NF-TS-4 NF-TS-5 NF-TS-6 |
Trans-sialidase, putative | HHD Tg mice | HLA-A*02:01 | (64) |
| FADKPDESTLS HTDKFNKKM |
KMP-1141-50 (K3) KMP-1158-66 (K4) |
Kinetoplastid membrane protein-11 (KMP-11) | A2.1/Kb Tg mice | HLA-A*02:01 | (41, 42) |
| YEIQYVDL RVVSFTQM | PFR-1164−171
PFR-3123–130 |
Paraflagellar rod component/paraflagellar rod protein | CL57BL/6 Cd8atm1Mak |
H-2Kb | (65) |
| GVPVDPSRV YLAAYALVGL |
P1 P7 |
Ribosomal P2 type protein/TcP2β | HHD Tg mice | HLA-A*02:01 | (37) |
| SLFGYRKL | Tcβ3 P1 | Adaptin AP-3 complex β3 subunit Tcβ3 | CL57BL/6 | H-2Kb | (66) |
| PAALFKEL | FCaBP p4 | Flagellar Ca+2 binding protein | CL57BL/6 | H-2Kb | (66) |
| ELTMYKQLL | LYT1 p5 | LYT-1 | CL57BL/6 | H-2Kb | (66) |
| SVPIRLLVL LGFQERNVL |
GFTKB-16 GFTKB-17 |
β-galactofuranosyl transferase | CL57BL/6 | H-2Kb | (33) |
| PSVRSSVPL VPLNKCNRL |
CRZPKB-5 CRZPKB-9 |
Cruzipain/cysteine peptidase | CL57BL/6 | H-2Kb | (33) |
| DSLTNLRAL RIPKVMQLV |
HSP7021255-263
HSP7021345-53 |
Heat shock protein-70 | A2/Kb Tg mice | HLA-A*02:01 | (47) |
| VVMACLVPA | Non-TS 2 | Cruzipain precursor/cruzipain/cysteine peptidase | HHD Tg mice | HLA-A*02:01 | (64) |
| TLLFQVLLL RIGDVCAEV LLFQVLLLC AVFDSVYSV TLMDFCPYI |
Non-TS 3 Non-TS 4 Non-TS 5 Non-TS 25 Non-TS 33 |
Surface protease GP63 | HHD Tg mice | HLA-A*02:01 | (64) |
| YVVSLLADT SVVSVFFLL |
Non-TS 6 Non-TS 29 |
Histone H3 | HHD Tg mice | HLA-A*02:01 | (64) |
| ALHSLVLFL SLFPFFFFV ALLPWLLVL FLIFRFMVL FMACHTNPI FLNQFGTRL |
Non-TS 7 Non-TS 9 Non-TS 13 Non-TS 17 Non-TS 18 Non-TS 26 |
Hypothetical protein | HHD Tg mice | HLA-A*02:01 | (64) |
| AVIPSTFPL | Non-TS 8 | ATP synthase | HHD Tg mice | HLA-A*02:01 | (64) |
| FLVDTIYSI | Non-TS 10 | Citrate synthase | HHD Tg mice | HLA-A*02:01 | (64) |
| YLDAVFYPL | Non-TS 11 | Pitrilysin-like metalloprotease | HHD Tg mice | HLA-A*02:01 | (64) |
| SLLCVISFI SMNAGLYLL |
Non-TS 12 Non-TS 24 |
Retrotransposon hot spot protein | HHD Tg mice | HLA-A*02:01 | (64) |
| LMNDVWFSL | Non-TS 16 | Heat shock protein 70 | HHD Tg mice | HLA-A*02:01 | (64) |
| MMHPFLCAL | Non-TS 19 | Surface protein ToIT | HHD Tg mice | HLA-A*02:01 | (64) |
| SMQEYRHMV | Non-TS 27 | Dynein light chain | HHD Tg mice | HLA-A*02:01 | (64) |
| QMLHNVASL | Non-TS 31 | Pumilio/PUF RNA binding protein | HHD Tg mice | HLA-A*02:01 | (64) |
| MMMTGRVLL | Non-TS 32 | Mucin-associated surface protein | HHD Tg mice | HLA-A*02:01 | (64) |
Underlined sequences are evaluated in more than one publication referenced.
ID from publication. (#) symbol refers to epitopes contained in epitopes recognized by T cells from Chagas disease patients.
Antigen name from NCBI database and the publication referenced.
Mouse models used for the analysis of T cell specificity. Cd8atm1Mak mice lack surface expression of CD8 due to the disruption of Lyt-2 (Cd8a), the gene coding for the T-cell surface glycoprotein CD8 α chain. TSKB20, TSK18 and TSK20/18 Tg mice express TSK20, TSK18 or both T. cruzi epitopes from TS proteins as self antigens.
MHC restriction is indicated in wild type (H2-K or H2-D) and Tg (HLA-A*02:01) mice. In assays with Tg mice, animals are immunized with the antigen and their splenocytes stimulated with APCs expressing HLA-A2.1 molecules pulsed with the same antigen.