Table 3. Composition of aggregation medium.
| Reagent | Amount for 50 ml | Supplier | Dilution factor | Product no. | Stock conc. | Final conc. |
|---|---|---|---|---|---|---|
| smNPC medium | 45 ml | |||||
| Polyvinyl alcohol (PVA, in water)* | 5 ml | Sigma-Aldrich | 10 | 363170 | 4% (w/v) | 0.4% (w/v) |
| Reagent | Amount for 50 ml | Supplier | Dilution factor | Product no. | Stock conc. | Final conc. |
|---|---|---|---|---|---|---|
| smNPC medium | 45 ml | |||||
| Polyvinyl alcohol (PVA, in water)* | 5 ml | Sigma-Aldrich | 10 | 363170 | 4% (w/v) | 0.4% (w/v) |