Table 5. Composition of maturation medium.
| Reagent | Supplier | Dilution factor | Product no. | Stock conc. | Final conc. |
|---|---|---|---|---|---|
| Basal medium | |||||
|
Activin A (day 6 only!) (in PBS + 0.1% (v/v) HSA) |
PeproTech | 2,000 | 120-14E | 10 μg/ml | 5 ng/ml |
|
BDNF (in PBS + 0.1% (v/v) HSA) |
PeproTech | 5,000 | 450-02 | 10 μg/ml | 2 ng/ml |
|
GDNF (in PBS + 0.1% (v/v) HSA) |
PeproTech | 5,000 | 450-10 | 10 μg/ml | 2 ng/ml |
|
TGFβ-3 (in PBS + 0.1% (v/v) HSA) |
PeproTech | 1,000 | 100-36E | 1 μg/ml | 1 ng/ml |
| dbcAMP (in water) | Sigma-Aldrich | 500 | D0627 | 50 mM | 100 μm |