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Evaluation of the robustness of the multiplex RT-LAMP assay. Variations in primer concentrations (0.75×, 0.5× and 0.4× of the optimal concentration) at 63°C (A) and 65°C (B). Positive RT-LAMP reactions were visually determined based on a color change from brown to green. NTC, negative non-template control; SPC, SARS-CoV-2 positive control.
