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. 2021 Jun 29;11:653616. doi: 10.3389/fcimb.2021.653616

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Copyright © 2021 Juscamayta-López, Valdivia, Horna, Tarazona, Linares, Rojas and Huaringa

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of temperature and R2, S4, and 8N1 primer sets on RT-LAMP assay performance. Error bars represent the standard deviations of the amplification time mean from independent experiments performed in duplicate. ***P < 0.001. Significance analyzed by one-way analysis of variance (ANOVA) (A). RT-LAMP assays using the R2, S4, and 8N1 primer sets for SARS-CoV-2 detection were considered positive when the solution color changed from brown to green (B). NTC, negative non-template control; SPC, SARS-CoV-2 positive control.