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. Author manuscript; available in PMC: 2021 Jul 13.
Published in final edited form as: Cell Host Microbe. 2019 Nov 13;26(5):650–665.e4. doi: 10.1016/j.chom.2019.10.011

Figure 3: The microbiotas of conventional and humanized mice display distinct signatures under repeated ciprofloxacin treatment.

Figure 3:

See also Figure S3.

(A) Schematic for treatment with two courses of ciprofloxacin.

(B,E) Culturable anaerobic and aerobic fecal densities in (B) humanized and (E) conventional mice treated with ciprofloxacin (treatment marked by colored areas) revealed that conventional mice responded similarly to both treatments, but humanized mice had a more robust second response. Error bars: S.E.M.

(C,F) Family-level composition in feces of (C) humanized and (F) conventional mice further demonstrated robustness of humanized mice to a second treatment.

(D,G,I) With differential resistance to antibiotics, resistant strains dominate during antibiotic treatment; other Bacteroides spp. expand after antibiotics. Relative abundance of the 10 most abundant Bacteroides ASVs in (D) ciprofloxacin-treated humanized mice, (G) ciprofloxacin-treated conventional mice and (I) streptomycin-treated humanized mice. B. sp: unknown Bacteroides spp.; Bc: B. caccae; Bs: B. salyersiae; Bf: B. fragilis; Bu: B. uniformis; Bi: B. intestinalis; Bv: B. vulgatus; Ba: B. acidifaciens. MICs are labeled above days of isolation, and colored by the appropriate ASV based on 16S Sanger sequencing. Treatment adenoted by shaded areas.

(H) Comparison of gene clusters and average nucleotide identity of Bv isolates revealed that day 0 (D0) isolate is different from isolates from other days, and that Bv isolates from conventional mice also cluster.