Figure 8. Analysis of pathological toxicity after treatment with JCo extract.

(A) HepG₂ cells were subcutaneously injected into the right flank of female nude mice (n=3). Mice were divided into an experimental and a control group (n=5), and both groups were subcutaneously treated with mineral oil (100 μg/ml), while only the experimental group also received JCo extract (200 mg/kg). Body weights were determined every 2 days. (B) After the killing of mice, organs were collected, fixed with 10% formalin, and stained with H&E to observe the tissue morphology under 400× magnification.