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. 2021 Jun 29;12:683588. doi: 10.3389/fendo.2021.683588

Figure 3.

Figure 3

Proposed method for processing fresh or frozen adrenal tumor tissue from patients with primary aldosteronism for mutational analysis. This approach varies from FFPE processing (Figure 1) by the method of tissue collection and the need to use CYP11B2 mRNA detection for confirmation of APA status. However, mutational analysis is similar using Sanger and/or next-generation sequencing to detect known and/or novel drivers of aldosterone production.