Table 2.
PCR primer sets for aldosterone-driver mutation hotspots.
| Gene | Exon | Primer Sequences | Amplicon Size (bp) | Reference | |
|---|---|---|---|---|---|
| KCNJ5 | 2 | Forward | GGACCATGTTGGCGACCAAGAGTG | 211 | (21) |
| Reverse | GACAAACATGCACCCCACCATGAAG | ||||
| ATP1A1 | 4 | Forward | ATTAACATCTGCTCGTGCAGCTGAG | 227 | |
| Reverse | CCATATGCTGAATTACAGAACTCAC | ||||
| ATP2B3 | 8 | Forward | TGTCTGCCATCACCGTCATCATC | 255 | (14) |
| Reverse | CCCAGTTTCCGAGTCTGTAAACAG | ||||
| CACNA1D | 8A | Forward | CCCACTCCTATGAGACCATC | 190 | |
| Reverse | TCTTGGCAACTGTCCTCAGG | ||||
| 16 | Forward | GGTGTGTGGCGTTGCCATTG | 253 | (29) | |
| Reverse | AACTGTTGCAGGGCTCCCA | ||||