Table 2.
Genotypes of T3 Camelina progenies revealed by Sanger sequencing following homoeolog-specific PCR.
| Line | FAD2-1 | FAD2-2 | FAD2-3 | Genotype | |||
|---|---|---|---|---|---|---|---|
| Zygosity | Mutation | Zygosity | Mutation | Zygosity | Mutation | ||
| EC#5-1-1 | hom. | 1i+1i | hom. | 1i+1i | WT | WT | aabbCC |
| EC#5-1-4 | hom. | 1i+1i | hom. | 1i+1i | het. | 1i | aabbCc |
| EC#5-1-5 | hom. | 1i+1i | bi. | 1i+5d | het. | 1i | aabbCc |
| EC#5-1-6 | hom. | 1i+1i | hom. | 1i+1i | WT | WT | aabbCC |
| EC#5-1-22 | hom. | 1i+1i | hom. | 1i+1i | het. | 1i | aabbCc |
| EC#5-1-24 | hom. | 1i+1i | bi. | 1i+5d | het. | 1i | aabbCc |
| EC#5-6-14 | bi. | 1i+3d | hom. | 5d+5d | het. | 1i | aa′bbCc |
| EC#5-6-18 | bi. | 1i+3d | hom. | 5d+5d | hom. | 1i+1i | aa′bbcc |
| EC#5-6-23 | hom. | 1i+1i | hom. | 1i+1i | hom. | 1i+1i | aabbcc |
| EC#5-6-24 | bi. | 1i+3d | hom. | 5d+5d | het. | 1i | aa′bbCc |
| EC#5-7-9 | bi. | 1i+3d | hom. | 1i+1i | het. | 1i | aa′bbCc |
| EC#5-7-19 | hom. | 1i+1i | hom. | 1i+1i | hom. | 1i+1i | aabbcc |
| EC#5-7-20 | hom. | 3d+3d | hom. | 1i+1i | hom. | 1i+1i | aabbcc |
| EC#5-7-21 | bi. | 1i+3d | hom. | 1i+1i | hom. | 1i+1i | aa′bbcc |
| EC#5-12-5 | hom. | 3d+3d | hom. | 1i+1i | hom. | 1i+1i | a′a′bbcc |
| EC#5-20-8 | hom. | 1i+1i | bi. | 1i+5d | WT | WT | aabbCC |
| EC#5-26-3 | bi. | 1i+3d | hom. | 1i+1i | WT | WT | aa′bbCC |
| EC#5-26-10 | bi. | 1i+3d | hom. | 1i+1i | het. | 1i | aa′bbCc |
| EC#5-26-20 | bi. | 1i+3d | hom. | 1i+1i | het. | 1i | aa′bbCc |
| EC#5-26-22 | hom. | 1i+1i | hom. | 1i+1i | hom. | 1i+1i | aabbcc |
| EC#5-26-24 | bi. | 1i+3d | hom. | 1i+1i | hom. | 1i+1i | aa′bbcc |
| EC#5-35-4 | bi. | 1i+3d | hom. | 5d+5d | het. | 1i | aa′bbCc |
| EC#5-35-15 | hom. | 1i+1i | hom. | 5d+5d | hom. | 1i+1i | aabbcc |
| EC#5-35-17 | hom. | 3d+3d | hom. | 5d+5d | het. | 1i | a′a′bbCc |
| EC#5-35-24 | bi. | 1i+3d | hom. | 5d+5d | hom. | 1i+1i | aa′bbcc |
| EC#5-44-3 | hom. | 3d+3d | bi. | 1i+5d | het. | 1i | a′a′bbCc |
| EC#5-44-11 | hom. | 3d+3d | bi. | 1i+5d | hom. | 1i+1i | a′a′bbcc |
| EC#5-44-15 | hom. | 3d+3d | hom. | 5d+5d | hom. | 1i+1i | a′a′bbcc |
| EC#5-44-20 | hom. | 3d+3d | hom. | 1i+1i | hom. | 1i+1i | a′a′bbcc |
| EC#5-44-24 | hom. | 3d+3d | bi. | 1i+5d | het. | 1i | a′a′bbCc |
1i, 3d, and 5d indicate 1 nt insertion, 3 nt deletion, and 5 nt deletion, respectively. Bi., het., and hom. represent biallelic, heteroallelic, and homoallelic mutants, respectively. Uppercase letters, lowercase letters, and lowercase letters with ′ in the Genotype column represent an intact CsFAD2 homoeolog, a nonsense CsFAD2 mutant gene, and a missense CsFAD2 mutant gene with a 3 nt deletion, respectively. A, B, and C in the Genotype column represent CsFAD2-1, CsFAD2-2, and CsFAD2-3, respectively.