Figure 8.

Diverse effects of Nrf1 and Nrf2 on those HCC-relevant proteins that are significantly correlated with the overall survival (OS). (A) The correlation analysis between of hepatocellular carcinoma (HCC) associated genes and the relevant OS rates of patients with HCC. (B) The protein expression levels of HCC-associated proteins in Nrf1α, TCF11, TCF11^ΔN and Nrf2 cell models were examined by Western blotting analysis of these experimental cell lines that had been induced with or without 1 μg/ml Tet for 12 h before being harvested. (C) Both wild type HepG2 and its derived Nrf1α^−/− cells were transfected with either Nrf1α or TCF11 expression plasmids, and then allowed for 24-recovery from transfection in the fresh medium before being subjected to Western blotting, to identify the protein levels of HCC-associated proteins. (D) Both wild type HepG2 and its derived Nrf2^−/− cells were transfected with an Nrf2 expression plasmid, and then allowed for 24-recovery from transfection in the fresh medium before being subjected to Western blotting. (E) MHCC97L or MHCC97H cells were transfected with each of Nrf1α, TCF11 and Nrf2 expression constructs, and then allowed for 24-recovery from transfection in the fresh medium before being examined by Western blotting to identify abundances of HCC-associated proteins as described above. The intensity of all the immunoblots was calculated and shown on the bottom (B–E).