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. 2021 Jun 29;11:668247. doi: 10.3389/fonc.2021.668247

Figure 3.

Figure 3

Inhibition of PI3K suppresses BCC cell growth and HH signaling. (A) Gli1 mRNA expression in ASZ001 cells treated with DMSO or varying concentrations of BKM120 or LY294002 (n = 3 experiments). dR, delta reporter gene normalized to passive reference dye. Error bar, SEM. Significance was determined by one-way ANOVA test. ***P < 0.001. (B, C) MTT assay of ASZ001 cells treated with DMSO or varying concentrations of (B) BKM120 or (C) LY294002 (n = 3 experiments). Abs, absorbance. Error bar, SEM. Significance was determined by two-way ANOVA test. *p < 0.05; ***p < 0.001. (D) Immunofluorescence staining of the indicated markers in ASZ001 cells treated with DMSO or BKM120. Scale bar, 200 µm. (E) Quantification of cleaved Casp3 signal (n = 4 experiments). (F) Quantification of Mki67 signal (n = 4 experiments). Error bars, SEM. Significance was determined by unpaired two-tailed t test. ***p < 0.001.