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. 2021 Jun 29;11:668247. doi: 10.3389/fonc.2021.668247

Figure 4.

Figure 4

PI3K inhibition suppresses murine BCC growth and stabilizes p21. (A) Hematoxylin and eosin staining of dorsal back skin collected from Ptchfl/fl; Gli1-CreERT2 mice treated with DMSO or BKM120. Tx, treatment. Scale bar, 50 μm. (B) Quantification of total tumor size per square area (n>250 tumors from 5 mice). Tx, treatment. (C) Immunofluorescence staining of indicated markers (red) and DAPI counterstain (blue) in Ptch1fl/fl; Gli1-CreERT2 tumors treated with DMSO or BKM120. Scale bar, 25 μm. (D) Quantification of immunofluorescence intensity of indicated markers (five points of measurement per animal, n=3 mice). AU, arbitrary unit. (E) Immunofluorescence staining of indicated markers (red) and DAPI counterstain (blue) in Ptch1fl/fl; Gli1-CreERT2 tumors treated with DMSO or BKM120. Scale bar, 25 μm. (F) Quantification of immunofluorescence intensity of indicated markers (five points of measurement per animal, n=3 mice). Error bars, SEM. Significance was determined by unpaired two-tailed t test. *p < 0.05; ***p < 0.001.