Table 2.

PD-L1 molecule numbers on SKOV3 or OVCAR3 cells 24 hours after exposure to primary T cells (10:1 E:T) and various IgGs and IgG BsAbs (0.1 nM). Columns with ‘fresh’ tumor cells were incubated for 24 hours with supernatants from the original redirected lysis assays cultures (T cells and original tumor cells removed by centrifugation), thus evaluating whether factors released into the supernatants of an active redirected lysis assay could induce PD-L1 upregulation on untreated tumor cells

Test article	PD-L1 molecules per cell surface (SKOV3 cells+T cells)	PD-L1 molecules per cell surface (fresh SKOV3 cells+24-hour supernatant)	PD-L1 molecules per cell surface (OVCAR3 cells+T cells)	PD-L1 molecules per cell surface (fresh OVCAR3 cells+24-hour supernatant)
IgG1 Cntrl	3500	7100	4500	1600
SP34 IgG1_N297Q	7700	5700	2000	1800
Pertuzumab IgG1	3900	3000	2000	1600
SP34+Pertuz IgGs	8300	6200	2200	1800
SP34/Pertuz IgG BsAb	29 300	29 400	5900	3000
SP34_F100fH/Pertuz BsAb	24 300	27 300	5000	2900

BsAb, bispecific antibody; PD-L1, Programmed cell death-ligand 1; scFV, Single chain variable fragment.