Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 12;9(7):e002503. doi: 10.1136/jitc-2021-002503

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

The effect of AB680 and programmed cell death protein 1 (PD-1) blockade on the in vivo AOM-DSS colorectal cancer (CRC) model. After sacrifice of mice as described in the Materials and methods section, (A) representative images for tumors in large intestines of mice for the AOM/DSS-induced control (n=8), AOM/DSS+AB680 (10 mg/kg) treated (n=8), AOM/DSS+PD-1 blockade (10 mg/kg) treated (n=8) and AOM/DSS+AB680 (10 mg/kg)+PD-1 blockade (10 mg/kg) treated group (n=8). (B) The diameters of all tumors in large intestines of mice for each group. (C) The number of tumors per mouse were measured for each group. Data are presented as mean ± SEM (t-test, NS, non-significant; *p<0.05; **p<0.01). (D) Percentages of CD4⁺ T cells and CD8⁺ T cells and the percentages of CD4⁺ Foxp3⁺ Tregs of large intestinal tissues from healthy and AOM-DSS murine models with or without AB680 or PD-1 blocker treatment. These results are representative from all independent experiments (n=8 per each group). The graphical results show (E) the percentages (%) of CD4⁺ Foxp3⁺ Treg per total T cells and (F) the percentages (%) of GzB⁺ CD8⁺ T cells of large intestinal tissues. Data are presented as mean ± SEM (t-test, NS, non-significant; ***p<0.001). (G) The histograms show the proliferating CD8⁺ T cells in the in vitro culture with or without ATP (20 µM) and AB680 (0.5 and 20 µM). These results are representative from three independent experiments. (H) The representative histograms show the mean fluorescence of intensity (MFI) for CD73 expression level of CD8⁺ T cells in the in vitro culture with or without ATP (20 µM) and AB680 (20 µM). These results are representative from three independent experiments. (I) The graphical result shows the percentages (%) of IFNγ⁺ CD8⁺ T cells after the in vitro culture with or without ATP (20 µM) and AB680 (0.5 and 20 µM) (n=6). Data are presented as mean±SEM (t-test, **p<0.01; ***p<0.001).