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. 2021 Jul 9;220(9):e202011133. doi: 10.1083/jcb.202011133

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© 2021 Kumar et al.

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Figure S1. — Generation of CEP90^−/−, MNR^−/−, and PCM1^−/− cell lines.(a–c) Sequence analysis of genomic DNA isolated from control and CEP90^−/− (a), MNR^−/− (b), and PCM1^−/− (c) RPE1 cell lines generated using CRISPR-Cas9. Insertions and deletions, and translation products resulting from genome editing are indicated. The protospacer adjacent motif (PAM) is indicated in green. (d) Immunoblot analysis of whole-cell lysates from control, CEP90^−/−, MNR^−/−, and PCM1^−/− RPE1 cell lines confirms loss of protein in mutant cell lines. The specific MNR band is indicated with an asterisk, and the top band is nonspecific.