Fig. 1.
Response of sockeye salmon to PRV infection. a Mean (line) and individual (dot) PRV RNA loads and infection prevalence (bars) measured at 0, 1, 4, and 9 weeks post-challenge (wpc). b Kaplan-Meier survival curves (left axis) of saline control (SC) and PRV-injected (PRV) sockeye (log-rank p = 0.32) and mean (box) ± SD hematocrit (right axis) at 1, 4, and 9 wpc; *p < 0.05 by 2-way ANOVA and Dunnett’s multiple comparison tests of arcsine-transformed hematocrit values. c Mean fold change (± SE) of interferon-stimulated gene (ISG) transcription measured as mean mx1, rsad2, and eif2ak2 mRNA transcripts relative to their expression in time-matched controls (SC); **p < 0.01 by 2-way ANOVA and Dunnett’s multiple comparison tests of corrected normalized relative quantities (CNRQ); minimum twofold change suggestive of biological relevance is shaded. d Correlation of ISG transcript abundance relative to log-PRV RNA load; Spearman r and associated p-value provided; mean (dotted line) ± SD (shaded) of SC ISG transcription suggestive of minimum threshold for biological relevance. e Correlation of blood hemoglobin concentration and f duration of excess post-exercise oxygen consumption (EPOCdur) relative to log-PRV RNA load; Spearman r and associated p-value provided; mean (dotted line) ± SD (shaded) of SC is indicated in both instances as suggestive of minimum threshold for biological relevance