Table 2.
Ticks collected from equids in selected districts of Khyber Pakhtunkhwa, Pakistan, between November 2018 and October 2019
| Districts | Tick species | Total no. collected ticks | Ticks processed for molecular analysesa | No. of ticks yielding rickettsial DNA | Host infested with Rickettsia-positive ticks |
|---|---|---|---|---|---|
| Upper Dir | Rhipicephalus. microplus | 11 | 4 N, 1 M, 3 F | 1 N | Mule |
| Bajaur | Rh. haemaphysaloides | 17 | 3 N, 1 M, 2 F | 1 N | Horse |
| Rh. microplus | 07 | 2 N, 1 F | 1 N | ||
| Mardan | Rh. haemaphysaloides | 51 | 2 N, 1 F | 1 N | Horse |
| Rh. microplus | 8 | 2 N, 1 F | 1 N | ||
| Rh. turanicus | 9 | 1 N, 1 F | |||
| Rh. sanguineus | 33 | 3 N, 1 M, 2F | |||
| Peshawar | Rh. microplus | 92 | 3 N, 3 F | 2 Nb | Donkey |
| Hyalomma anatolicum | 11 | 3 N, 1 M, 3 F | |||
| Charsadda | Rh. haemaphysaloides | 85 | 3 N, 2 F | 1 N | Horse |
| Rh. microplus | 10 | 2 N, 1 M, 1 F | 1 N | ||
| Malakand | Rh. microplus | 17 | 1 N, 1 F | 1 N | Horse |
| Rh. turanicus | 3 | 2 N, 1 M, 1 F | |||
| Swabi | Rh. microplus | 82 | 2 N, 1 F | 1 F | Horse |
| Rh. turanicus | 14 | 3 N, 1 M, 2 F | |||
| Nowshera | Rh. microplus | 87 | 2 N, 3 F | 1 N | Mule |
| Rh. turanicus | 13 | 2 N, 1 M, 3 F | |||
| Buner | Rh. microplus | 27 | 6 N, 1 M, 5 F | 1 F | Donkey |
| Total | 550 | 91 (46 N, 9 M, 36 F) | 13 (14%) |
aPCR assays targeting the tick 16S rRNA gene and the rickettsial gltA, and ompA gene; N nymphs, M males, F females
bBoth nymphs of Rh. microplus were from the same donkey