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. Author manuscript; available in PMC: 2021 Jul 13.
Published in final edited form as: Cell Rep. 2021 Jun 22;35(12):109205. doi: 10.1016/j.celrep.2021.109205

Figure 2. DHX15 is required for IFN-β, IFN-λ3, and IL-18 production in human HT-29 IECs upon enteric RNA virus infection.

Figure 2.

(A–I) ELISA of IFN-β (A, D, and G), IFN-λ3 (B, E, and H), and IL-18 (C, F, and I) production from human HT-29 IECs with the indicated shRNA after a 20-h infection with enteric RNA viruses, including simian rotavirus SA-11 strain (A–C) and reovirus T3D strain (D–F), or DNA virus HSV-1 KOS strain (G–I) at a multiplicity of infection (MOI) of 10. Mock, scrambled shRNA (sh-Ctrl)-treated human HT-29 IECs without virus infection. Each circle represents an individual independent experiment, and small solid black lines indicate the average of triplicates.

(J–L) Quantification of expression of rotavirus NSP5 gene (J), reovirus S4 gene (K), and HSV-1 VP16 gene (L) relative to GAPDH in human HT-29 IECs infected by rotavirus (J), reovirus (K), or HSV-1(L) as in (A)–(I). Data are represented as means ± SEMs. NS, p > 0.05, ***p < 0.001 (unpaired t test).