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. Author manuscript; available in PMC: 2021 Jul 13.
Published in final edited form as: Cell Rep. 2021 Jun 22;35(12):109205. doi: 10.1016/j.celrep.2021.109205

Figure 3. DHX15 positively regulates production of IFN-β, IFN-λ3, and IL-18 in mouse primary IECs upon enteric RNA virus infection.

Figure 3.

(A–I) ELISA of IFN-β (A, D, and G), IFN-λ3 (B, E, and H) and IL-18 (C, F, and I) production in mouse primary IECs from wild-type Dhx15fl/fl and Dhx15IEC-KO mice after a 20-h infection with enteric RNA viruses, including rotavirus EW strain (A–C) and reovirus T3D strain (D–F), or DNA virus HSV-1 KOS strain (G–I) at a MOI of 10. Mock, cells without virus infection. Each circle represents an individual independent experiment, and small solid black lines indicate the average of triplicates.

(J–L) Quantification of expression of rotavirus NSP5 gene (J), reovirus S4 gene (K), and HSV-1 VP16 gene (L) relative to GAPDH in mouse primary IECs from wild-type Dhx15fl/fl and Dhx15IEC-KO mice infected by rotavirus (J), reovirus (K), or HSV-1(L) as in (A)–(I). Data are represented as means ± SEMs. NS, p > 0.05, ***p < 0.001 (unpaired t test).