KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-DHX15 | Abcam | Cat#ab254591; RRID: AB_2892059 |
| anti-MAVS | Cell Signaling Technology | Cat#3993S; RRID:AB_823565 |
| anti-STING | Cell Signaling Technology | Cat#13647S; RRID:AB_2732796 |
| anti-DHX9 | Abcam | Cat#ab26271; RRID:AB_777725 |
| anti-RIG-I | Cell Signaling Technology | Cat#3743S; RRID:AB_2269233 |
| anti-MDA5 | Cell Signaling Technology | Cat#5321S; RRID:AB_10694490 |
| anti-NLRP6 | Sigma-Aldrich | Cat#SAB1302240; RRID:AB_2750643 |
| anti-NLRP9 | Novus Biologicals | Cat#NBP2-24661; RRID:AB_2892061 |
| anti-GAPDH | Sigma-Aldrich | Cat#G9295; RRID:AB_1078992 |
| anti-β-actin | Sigma-Aldrich | Cat#A3854; RRID:AB_262011 |
| APC/Cyanine7 anti-mouse CD45 | BioLegend | Cat#103116; RRID:AB_312981 |
| PE anti-mouse/human CD324 (E-Cadherin) | BioLegend | Cat#147304; RRID:AB_2563040 |
| PE/Cyanine7 anti-mouse CD326 (Ep-CAM) | BioLegend | Cat#118216; RRID:AB_1236471 |
| FITC anti-mouse CD3 | BioLegend | Cat#100204; RRID:AB_312661 |
| PE/Cyanine7 anti-mouse CD4 | BioLegend | Cat#100528; RRID:AB_312729 |
| PerCP/Cyanine5.5 anti-mouse CD8a | BioLegend | Cat#100734; RRID:AB_2075238 |
| APC anti-mouse CD19 | BioLegend | Cat#152410; RRID:AB_2629839 |
| PE anti-mouse NK-1.1 | BioLegend | Cat#108708; RRID:AB_313395 |
| Bacterial and virus strains | ||
| Reovirus T3D strain | ATCC | Cat#VR-824 |
| Rotavirus EW strain | Dr. Harry B. Greenberg (Stanford University) | Dr. Harry B. Greenberg (Stanford University) |
| Rotavirus SA-11 strain | ATCC | Cat#VR-1565 |
| Herpes simplex virus 1(HSV-1) KOS strain | ATCC | Cat#VR-1493 |
| Vesicular stomatitis virus (VSV) Indiana strain | ATCC | Cat#VR-1238 |
| Influenza A virus A/PR/8/34 strain | ATCC | Cat#VR-95 |
| Biological samples | ||
| Collagenase D | Sigma-Aldrich | Cat#11088882001 |
| DNase I | Sigma-Aldrich | Cat#10104159001 |
| Chemicals, peptides, and recombinant proteins | ||
| UltraPure 0.5M EDTA, pH 8.0 | Invitrogen | Cat#15575020 |
| Percoll | GE Healthcare | Cat#17089101 |
| Critical commercial assays | ||
| Human IFN-β ELISA kit | PBL InterferonSource | Cat#41415-1 |
| Mouse IFN-β ELISA kit | PBL InterferonSource | Cat#42410-1 |
| Human IFN-lambda 3 (IFN-λ3) ELISA kit | R&D systems | Cat#D28B00 |
| Mouse IFN-lambda 3 (IFN-λ3) ELISA kit | R&D systems | Cat#DIY1789B-05 |
| Human IL-18 ELISA kit | R&D systems | Cat#DY318-05 |
| Mouse IL-18 ELISA kit | R&D systems | Cat#DY7625-05 |
| Human IL-6 ELISA kit | R&D systems | Cat#DY206-05 |
| Human TNF-α ELISA kit | R&D systems | Cat#DY210-05 |
| RNeasy Mini Kit (250) | QIAGEN | Cat#74106 |
| iScript cDNA Synthesis Kit | Bio-Rad | Cat#1708891 |
| iTaq Universal SYBR Green Supermix | Bio-Rad | Cat#1725125 |
| Zombie Aqua Fixable Viability Kit | BioLegend | Cat#423102 |
| Poly(I:C) HMW | InvivoGen | Cat#tlrl-pic-5 |
| Poly(dG:dC) | InvivoGen | Cat#tlrl-pgcn |
| Experimental models: cell lines | ||
| Human intestinal epithelial cells (IECs) line HT-29 | ATCC | Cat#HTB-38 |
| HEK293T cells | ATCC | Cat#CRL-3216 |
| L929 cells | ATCC | Cat#CCL-1 |
| Experimental models: organisms/strains | ||
| Dhx15fl/fl conditional knockout mice | This paper | Taconic-Artemis |
| Villin-Cre transgenic mice | The Jackson Laboratory | Stock No: 021504 |
| IEC-specific Dhx15-knockout
mice,
Dhx15fl/fl; Villin-Cre (Dhx15IEC-KO) |
This paper | This paper |
| Oligonucleotides | ||
| shRNA for human DHX15 | Horizon Discovery | clone ID: TRCN0000000009 |
| shRNA for human MAVS | Horizon Discovery | clone ID: TRCN0000146651 |
| shRNA for human STING | Horizon Discovery | clone ID: TRCN0000161052 |
| shRNA for human RIG-I | Horizon Discovery | clone ID: TRCN0000151446 |
| shRNA for human MDA5 | Horizon Discovery | clone ID: TRCN0000050852 |
| See Table S1 for primer sequences | This study | N/A |
| Recombinant DNA | ||
| HA-DHX15 | This paper | This paper |
| HA-pro-IL-18 | SinoBiological | Cat#MG50073-CY |
| Myc-ASC | Addgene | Cat#73952 |
| Flag-Caspase-1 | Addgene | Cat#21142 |
| Flag-NLRP6 | Dr. Gabriel Núñez (University of Michigan) | Dr. Gabriel Núñez (University of Michigan) |
| Software and algorithms | ||
| Prism (version 8) | GraphPad Software | https://www.graphpad.com; RRID:SCR_002798 |
| FlowJo (version 10) | BD Biosciences | https://www.flowjo.com/ |
| Office 365 | Microsoft | https://www.office.com/ |