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. 2021 Jul 12;22:206. doi: 10.1186/s13059-021-02424-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The spatial distribution of replication origins within the TADs at the G1/S transition is dependent on CTCF, cohesin, and transcription. a Representative STORM images of origins (purple) in TAD1 (green) after treatment of cells with the indicated siRNAs. Conventional images indicate the concentration of CTCF (cyan) or cohesin (yellow) in the nucleus. b Left panel, efficiency of RNAi indicated by fluorescence of CTCF or cohesion. Right panel, barycenter distances between high-efficiency or low-efficiency origins in TAD1 after treatment of cells with the indicated siRNAs. Portions of the two signals that overlap are shown in white. c Representative STORM images of origins (purple) in TAD1 (green). Left: no DRB. Right: with DRB. d Barycenter distances between high-efficiency/low-efficiency origins and TAD1 with or without DRB treatment. e Radius of TAD1 treated with or without DRB. For lines and statistics in b, d, and e, see the description in the legend of Fig. 1 (n ≥ 10 cells). 3D results are shown in Fig. S13