Table 3:

Media composition for adult mouse cardiomyocyte plating and culture.

Plating media composition, pH 7.4
	Working Concentration	Molecular Wt.	Required amount
Culture media without Blebbistatin			50 mL
BDM	10 mM	101.105 g/mol	50.55 mg
FBS	5%		2.5 g
Culture media composition, pH 7.4
Reagent	Working Concentration	Molecular Wt.	Required amount
DMEM	1X		250 mL
Insulin	1 μg/mL		0.25 mg
Transferrin	0.55 μg/mL		0.138 mg
Selenium	0.5 ng/mL		0.125 μg
Penicillin (U/mL)-streptomycin (g/mL)	100-100		2.5 mL
HEPES	10 mM	238.3012 g/mol	595.753 mg
*FBS	10%		25 mL
*BSA	0.20%		0.5 g
#Blebbistatin	25 μM	292.338 g/mol	1.8271 mg

^*Use either of them, as per the experimental requirement.

^#Aliquot the culture media to prepare plating media before adding Blebbistatin.

NOTE: Prepare 200 mL of culture media.

NOTE: Prepare 50 mL of plating media.