Figure 2.

Mutating the VEGFA +157 enhancer leads to VEGFA exons 6a and 7 exclusion and to slower growth of these cells. (A andB) RNA was extracted from WT K562 cells and enhancer mutated clones 9 and 26 and analyzed by real-time PCR for total mRNA amount of VEGFA relative to CycloA and hTBP reference genes (A) and for VEGFA₁₂₁ and VEGFA₁₈₉ relative to VEGFA total mRNA amount. PSI was calculated by VEGFA₁₈₉ relative to VEGFA₁₂₁ (B). Plots represents the mean of three independent experiments and ± SD (*P< 0.05; **P< 0.01, ***P< 0.001). (C) WT K562 cells and enhancer mutated C9 and C26 were seeded and counted every day. Plot represents the mean of three independent experiments done with three replicates and ± SD (*P< 0.05, Student’s t test).