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. 2021 Jul 13;3(3):zcab029. doi: 10.1093/narcan/zcab029

Figure 3.

Figure 3.

VEGFA +157 enhancer promotes inclusion of VEGFA exons 6a and 7. (A andB) K562 cells were transfected with either dCas9-p300 core (mut) or dCas9-p300 core (WT) with four gRNAs targeted to the VEGFA promoter or +157 enhancer or with a single gRNA targeting each of four VEGFA enhancers marked in Figure 1B for 30 h. Total RNA was extracted and analyzed by real-time PCR for total mRNA of VEGFA relative to CycloA and hTBP reference genes (A) and for VEGFA121 and VEGFA189 relative to VEGFA total mRNA amount. PSI was calculated by VEGFA189 relative to VEGFA121 (B). Values are expressed as dCas9-p300 core (WT) relative to dCas9-p300 core (mut) and horizontal broken lines indicate no change between dCas9-p300 core (WT) relative to dCas9-p300 core (mut). Plots represents the mean of three independent experiments and ± SD (*P< 0.05; **P< 0.01).