Figure 4.

CML patients have greater inclusion of VEGFA exons 6a and 7 at diagnosis. RNA was extracted from peripheral blood samples collected from 12 CML patients at diagnosis (BCR-ABL positive, red circles, 1 sample per patient) and during remission (BCR-ABL negative, green triangles, 1–3 samples per patient). RNA was analyzed by real-time PCR for total mRNA amount of VEGFA and VEGFA +157 eRNA relative to CycloA and hTBP reference genes and for VEGFA₁₂₁ and VEGFA₁₈₉ relative to VEGFA total mRNA amount. PSI was calculated by VEGFA₁₈₉ relative to VEGFA₁₂₁.