Table 1.
Summary of protein diversity in large renal tubular extracellular vesicles
| Location | Category | UniProt/Swiss-Prot/GenPept Accession | Protein Symbol | Scan Count | Peptide Count | Entrez Gene Name |
|---|---|---|---|---|---|---|
| Cytoplasmic | Enzymes | P19468 | GSH1 | 25 | 260 | Glutamate-cysteine ligase catalytic subunit |
| P04764 | ENOA | 17 | 139 | Enolase 1 | ||
| P07632 | SODC | 8 | 109 | SOD1 | ||
| Ion channels | P81155 | VDAC2 | 10 | 44 | Voltage dependent anion channel 2 | |
| Q9Z2L0 | VDAC1 | 6 | 22 | Voltage dependent anion channel 1 | ||
| Kinases | Q4KLZ6 | TKFC | 16 | 74 | Triokinase and FMN cyclase | |
| P16617 | PGK1 | 10 | 40 | Phosphoglycerate kinase 1 | ||
| P07379 | PCKGC | 8 | 30 | Phosphoenolpyruvate carboxykinase 1 | ||
| Peptidases | P00758 | KLK1 | 8 | 165 | Kallikrein 1 | |
| P00787 | CATB | 12 | 147 | Cathepsin B | ||
| P16675 | PPGB | 10 | 125 | Cathepsin A | ||
| Phosphatases | P25113 | PGAM1 | 8 | 36 | Phosphoglycerate mutase 1 | |
| P20611 | PPAL | 8 | 27 | Acid phosphatase 2, lysosomal | ||
| Q91YE9 | 5NT1B | 8 | 18 | 5′-Nucleotidase, cytosolic 1B | ||
| Transporters | P55054 | FABP9 | 10 | 60 | Fatty acid binding protein 9 | |
| P19511 | AT5F1 | 3 | 59 | ATP synthase peripheral stalk-membrane subunit b | ||
| P10719 | ATPB | 14 | 44 | ATP synthase F1 subunit β | ||
| Extracellular | Cytokines | P08721 | OSTP | 7 | 86 | Secreted phosphoprotein 1 |
| Q9CPT4 | MYDGF | 3 | 14 | Myeloid-derived growth factor | ||
| P06684 | CO5 | 4 | 12 | Complement C5 | ||
| Growth factors | P07522 | EGF | 44 | 590 | EGF | |
| P01015 | ANGT | 15 | 189 | Angiotensinogen | ||
| P23785 | GRN | 7 | 98 | Granulin precursor | ||
| Other proteins | P17475 | A1AT | 40 | 2659 | Serpin family A member 1 | |
| P05545 | SPA3K | 32 | 2252 | Serine protease inhibitor A3K precursor | ||
| P27590 | UROD | 24 | 1724 | Uromodulin | ||
| Peptidases | Q01177 | PLMN | 58 | 1172 | Plasminogen | |
| P01026 | CO3 | 78 | 949 | Complement C3 | ||
| P06866 | HPT | 24 | 401 | Haptoglobin | ||
| Transporters | P02770 | ALBU | 9 | 16,638 | Albumin | |
| P12346 | TRFE | 65 | 3405 | Transferrin | ||
| P14046 | MUG1 | 74 | 1583 | Murinoglobulin 1 | ||
| Plasma membrane | Enzymes | P07314 | GGT1 | 21 | 324 | γ-Glutamyltransferase 1 |
| P97675 | ENPP3 | 10 | 23 | Ectonucleotide pyrophosphatase/phosphodiesterase 3 | ||
| Q8CFN2 | CDC42 | 3 | 19 | Cell division cycle 42 | ||
| G protein–coupled receptors | Q3KRC4 | GPRC5C | 2 | 8 | G protein–coupled receptor class C group 5 member C | |
| Ion channels | Q9Z0W7 | CLIC4 | 10 | 64 | Chloride intracellular channel 4 | |
| Q9Z0Y8 | CACNA1I | 2 | 7 | Calcium voltage-gated channel subunit α1 I | ||
| Q91YD4 | TRPM2 | 2 | 6 | Transient receptor potential cation channel subfamily M member 2 | ||
| Kinases | Q01279 | EGFR | 4 | 9 | EGF receptor | |
| Other proteins | P31977 | EZRI | 28 | 241 | Ezrin | |
| Q9R0T4 | CADH1 | 13 | 204 | Cadherin 1 | ||
| Q9JJ19 | NHRF1 | 25 | 131 | SLC9A3 regulator 1 | ||
| Peptidases | P15684 | AMPN | 30 | 418 | Alanyl aminopeptidase, membrane | |
| P07861 | NEP | 34 | 361 | Membrane metalloendopeptidase | ||
| P14740 | DPP4 | 31 | 289 | Dipeptidyl peptidase 4 | ||
| Transmembrane proteins | P07151 | B2MG | 8 | 143 | β-2-Microglobulin | |
| O70244 | CUBN | 17 | 58 | Cubilin | ||
| Q63257 | IL4RA | 4 | 48 | IL-4 receptor | ||
| Transporters | P98158 | LRP2 | 106 | 651 | LDL receptor-related protein 2/megalin | |
| Q64319 | SLC3A1 | 31 | 427 | Solute carrier family 3 member 1 | ||
| Q9JJ40 | NHRF3 | 20 | 152 | PDZ domain-containing 1 | ||
| P29975 | AQP1 | 8 | 151 | Aquaporin 1 | ||
| P15083 | PIGR | 21 | 134 | Polymeric Ig receptor | ||
| Q62687 | S6A18 | 12 | 86 | Solute carrier family 6 member 18 | ||
| Q63424 | S15A2 | 16 | 59 | Solute carrier family 15 member 2 | ||
| Q9WTW7 | S23A1 | 11 | 43 | Solute carrier family 23 member 1 | ||
| Q80W57 | ABCG2 | 9 | 33 | ATP binding cassette subfamily G member 2 | ||
| P50516 | VATA | 9 | 32 | ATPase H+ transporting V1 subunit A |
This table summarizes the three most abundant proteins (by scan count and if present) within each functional category (type) of select cellular locations identified by Ingenuity Pathway Analysis. The ten most abundant transport proteins in the plasma membrane are indicated. UniProt accession numbers, protein symbols, and Entrez gene name are also provided. See Supplemental Table 1 for a complete list of Ingenuity Pathway Analysis–identified protein location/types. SLC9A3, sodium-hydrogen antiporter 3; FMN, flavin mononucleotide; H+, hydrogen ion.