Figure 7.

HFD-Feeding Impairs Mitochondrial ADP Sensitivity and Attenuates the Ability of ADP to Suppress ROS Emission in Skeletal Muscle. (A–C) While maximal mitochondrial ROS emission rates were higher in obese mice compared to lean controls, HFD further impaired redox balance by attenuating the ability of ADP to suppress mitochondrial ROS (increased IC50) in both genotypes. (D, E) 4HNE protein content in RG was increased in both genotypes following HFD-feeding. (F, G) Mitochondrial ADP sensitivity was impaired following HFD in both genotypes. Data expressed as mean ± SEM of results obtained from n = 5–8 per genotype/diet (two-way ANOVA with LSD post hoc). IC50, half-maximal inhibitory concentration; JH₂O₂, rate of hydrogen peroxide production; JO₂, oxygen consumption; L, lean mice; Ob, obese mice; P + M+S, pyruvate + malate + succinate.