Skip to main content
. 2021 Jul 1;148(13):dev196519. doi: 10.1242/dev.196519

Fig. 2.

Fig. 2.

Compressive stress regulates tissue compaction through BMP signaling. (A) Percentage of cells positive for pSmad1 and pSmad5 in HH25 cushions cultured under osmotic stress for 24 h, n=4 or 5, data are mean±s.d. (B) Compaction trend for compressive stress combined with the Alk2/3 inhibitor LDN. (C) Compaction trend for BMP treatment combined with the Alk2/3 inhibitor LDN, n=4 or 5 cushions per condition, data are mean±s.e.m. (D) Representative immunofluorescence images stained for pSmad1/5 of HH25 cushions cultured under osmotic stress over 24 h. T, tensile stress; U, unloaded; C, compressive stress. Arrowhead indicates an example of a pSmad1/5-positive cell. Scale bar: 50 μm, **P<0.01 [ANOVA with Tukey's post-hoc test (A,C) and a two-tailed unpaired t-test (C)].