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. 2021 Jul 1;148(13):dev196519. doi: 10.1242/dev.196519

Fig. 3.

Fig. 3.

MEK-ERK is involved in the tissue growth driven by BMP and by compressive stress. (A) Compaction trend for compressive stress combined with the MEK inhibitor U0126. (B) Compaction trend for BMP treatment combined with the MEK inhibitor. (C) Percentage of cells positive for pERK in HH25 cushions cultured under osmotic stress, and cultured with BMP and MEK inhibitors. (D) Representative immunofluorescence images stained for pERK of HH25 cushions cultured under osmotic stress and cultured with a MEK inhibitor. T, tensile stress; U, unloaded; C, compressive stress. Scale bar: 50 μm, n=4-6 cushions per condition per three independent experiments, data are mean±s.e.m., *P<0.05, **P<0.01 [ANOVA with Tukey's post-hoc test (C) and a two-tailed unpaired t-test (A,B)].