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Periplasmic view of TolC in its resting, closed conformation (PDB ID 1EK9) and after full dilation of its aperture upon engagement with the PAP protein as seen in the assembled tripartite complex PDB ID 5NG5. Structures are shown as cartoon view, and aspartate D374 is shown as ball and sticks. Distances between hydroxyl groups of the carboxylic acid of D374 are measured.
