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. 2021 Jul 9;134(13):jcs253443. doi: 10.1242/jcs.253443

Fig. 4.

Fig. 4.

Mfn2 regulates calcium exchange-related MAM proteins in stable SH-SY5Y cells with manipulated Mfn2 expression. (A,B) Representative western blot (A) and quantitative analysis (B) of MAM proteins in the total lysates of negative control (NC) and Mfn2 KO SH-SH5Y cells. (C,D) Representative western blot (C) and quantitative analysis (D) of MAM proteins in the total lysates of empty vector (EV) and Mfn2 OE SH-SH5Y cells. The relative protein levels were normalized to actin. (E-H) Representative western blots (E,G) and quantitative analysis (F,H) of MAM proteins from the MAM fraction of NC and Mfn2 KO SH-SY5Y cells (E,F), and EV and Mfn2 overexpressing (OE) cells (G,H). Data are mean±s.e.m. of three independent experiments. *P<0.05, **P<0.01, ***P<0.001 (unpaired two-tailed Student's t-test). The relative protein levels were normalized to the protein levels of corresponding pure mitochondria with HSPD1 loading control.