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. 2021 Jul 8;134(13):jcs258464. doi: 10.1242/jcs.258464

Fig. 1.

Fig. 1.

KIF7 is regulated by auto-inhibition. (A) Domain organization of full-length KIF7. The position of truncation at amino acid 558 is indicated on top. CC, coiled coil; black box, CC prediction >90%; gray box, CC prediction >80%, according to COILS software. (B-D) COS-7 cells expressing mCit-tagged full-length (B) or truncated (1-558) (C) versions of KIF7 were fixed and stained with an antibody against β-tubulin to mark microtubules and with DAPI to mark the nucleus. The white boxed region is shown on the right with separate channels for KIF7-mCit and microtubules. (D) Quantification of microtubule (MT) binding. n=136 (KIF7) and 88 [KIF7(1-558)] cells across at least three independent experiments. (E-G) NIH-3T3 cells expressing mCit-tagged full-length (E) or truncated (1-558) (F) versions of KIF7 were fixed and stained with antibodies against β-tubulin to mark microtubules, Arl13b to mark the primary cilium, pericentrin to mark the basal body and DAPI to mark the nucleus. The white boxed region is shown to the right with separate channels for KIF7-mCit and microtubules. The primary cilium in the yellow boxed region is shown on the far right with separate images of KIF7 and the cilium markers. Purple arrowheads indicate localization along the cilium shaft. (G) Quantification of cilium localization. n=46 (KIF7) and 25 [KIF7(1-558)] cells across at least three independent experiments. Scale bars: 10 µm (B,C,E,F); 1 µm (E,F, far right panels).