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. 2021 Jun 29;14:656740. doi: 10.3389/fnmol.2021.656740

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Copyright © 2021 Zieminska, Ruszczynska, Augustyniak, Toczylowska and Lazarewicz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Box and Whiskers plot of changes in Cu (A), Zn (B), and Cu/Zn (C) levels in male rats in parts of brain: hippocampus, cerebellum and cerebral cortex, measured using the ICP-MS method. The obtained results are expressed in concentration μM/kg wet tissue (A,B). The number of animals used in this experiment was: n = 15 – control group, n = 12 – VPA-treated rats, and n = 12 – THAL-treated animals; (*) represents results statistically significant vs. control, p < 0.05, two-way ANOVA followed by Holm-Sidak corrections; box containing 50% of results that fell in the range between 25% (bottom line) and 75% (top line) – IQR (InterQuartile Range), (–) median value, whiskers – min and max values, (•) – outliers (±1.5 × IQR), (∘) – individual sample.