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| Instrument | Condition |
|---|---|
| Column | Waters AccQ-Tag (Waters) |
| Mobile phase | Waters AccQ-Tag eluent A, 60% Acetonitrile (pH 5.02) |
| Detector (detection) | Waters Dual λ Absorbance Detector W2487 (280 nm) |
| Flow rate | 1 mL/min |
| Injection volume | 10 μL |
