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Western blot. Western immunoblot for prolactin and β-actin protein bands in MCF7 cells transfected with prolactin (PRL) (wild-type; WT), PRL p.Arg220Ter (SG220), 50:50 PRL WT:PRL p.Arg220Ter (WT:SG220), or p.Lys218Ter (SG218). p.Arg220Ter and p.Lys218Ter run at a lower molecular weight and there is little detectable PRL p.Arg220Ter in the 50:50 PRL WT:PRL p.Arg220Ter. SG, stop gain.
