Figure 6. Ensemble-specific activity dynamics are maintained across days after learning.

(A) Cue discrimination scores (auROC; CS+ vs. CS-) showing similar behavior across days after learning. Data presented as mean ± SEM. (B) Correlation plots displaying the change in activity during CS+ and CS- trials for all tracked neurons during two imaging sessions late in learning. These responses were highly correlated (*p-values < 0.001). (C) Activity heat maps for all tracked neurons separated by cue (columns) and day (rows) reveal similar population dynamics across days after learning. (D–E) Activity heat maps for each ensemble separated into CS+ trials (D) and CS- trials (E) confirm similar activity patterns across days after learning.

Figure 6—figure supplement 1. Correlations in activity across sessions after learning are not due to similarities in activity across neurons within each field of view (FOV).

Correlation plot displaying mean responses (baseline vs. cue/reward period) of all tracked neurons during CS+ and CS- trials across two sessions after learning, but with tracking IDs shuffled within each FOV to cause inaccurate cell tracking. Lack of correlation (as compared to Figure 6B) suggests neuron-to-neuron correlations in activity were present within the dataset rather than general correlations in activity within each FOV.