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. 2021 Jun 29;11:672448. doi: 10.3389/fcimb.2021.672448

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Copyright © 2021 Callejas-Hernández, Herreros-Cabello, del Moral-Salmoral, Fresno and Gironès

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Strategy followed to confirm sequence and circularity of the Y strain minicircles. (B) PCR products of nine minicircles of the Y strain. (C) PCR products with the primers designed against the Y strain, tested in Bug2148 strain.