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. 2021 Jul 9;16:4739–4753. doi: 10.2147/IJN.S313140

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© 2021 Sil et al.

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Design concept of the rapid flow-through dot-blot immunoassay (FT-DBA). The gold nanoparticle (AuNP) with antibody conjugated onto the surface can bind to the SARS-CoV-2 antigen. At the same time, the AuNP with goat anti-mouse within the same solution can bind to mouse polyclonal antibody, which is the control dot.