Table 1.
Primary antibodies used.
| Antibody | Species | Detection | Dilution Factor | Catalog |
|---|---|---|---|---|
| GFP | Mouse | GFP+ GBM cells | 1:500 | Abcam (ab1218) |
| Human Nuclei (HuNu) | Mouse | Human GBM cells | 1:200 | Millipore (MAB1281) |
| Ki67 | Rabbit | Proliferating cells | 1:200 | Thermo (RM-9106-S0) |
| Mouse | Novocastra (NCL-L-Ki67-MM1) | |||
| phosphohistone H3 (pH3) | Rabbit | Proliferating cells | 1:200 | Cell Signaling (9701S) |
| GFAP | Rabbit | Astrocytic cells | 1:200 | Dako (Z0334) |
| SOX2 | Rat | Undifferentiated cells | 1:500 | Thermo (14-9811-82) |
| OLIG2 | Rabbit | Oligodendrocyte precursors | 1:500 | Millipore (AB9610) |
| Cleaved caspase-3 (Asp175) | Rabbit | Apoptotic cells | 1:200 | Cell Signaling (9661) |
| Doublecortin (DCX) | Goat | Neuroblasts | 1:200 | Santa Cruz (SC-8066) |