Fig. 4.

Suppression of the action of L. rhamnosus EH8 plus mycelium to reduce the IL-6 and PDE4B by Ffar2 inhibition. ICR mice were given GLPG-0974, a Ffar2 antagonist, or its DMSO solvent as a control by gastric gavage every day for 3 days, then subsequently fed with L. rhamnosus EH8 plus fungal mycelium every day. Two weeks after feeding, mice were intranasally inoculated with SARS-CoV-2 membrane glycoprotein (100 µg/60µL PBS per mouse). The mRNA expression of PDE4B (A) and levels of IL-6 (B) in BALF were detected by RT PCR and ELISA, respectively. Data are the mean ± SD of experiments performed in triplicate. ***p < 0.001 (two-tailed t-test).