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. 2021 Jun 24;23(7):692–703. doi: 10.1038/s41556-021-00694-x

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, Mid-streak-stage FVF/SCF embryos stained for Venus (Foxa2), E-cadherin and RFP (Sox17). The blue dashed line indicates the primitive streak (PS). a, anterior; d, distal; DE, definitive endoderm; p, posterior; pr, proximal. b, Immunohistochemistry of a mid-streak-stage FVF embryo stained for Venus (Foxa2), Snail1 and T. The blue dashed lines in the insets mark FVF^low epiblast progenitors (EPs) and the red dashed lines mark FVF^high transitory progenitors (TPs). The yellow arrowhead marks AME cells that synthesize Foxa2, T and Snail1. The horizontal white dashed line indicates the border of Snail1 expression. MES, mesoderm; TP, transitory progenitor; VE, visceral endoderm. c, Mid-streak-stage FVF embryo immunostained for Venus (Foxa2; white), N-cadherin and E-cadherin. The blue asterisk (rightmost image inset in white dashed box) and inset blue dashed line (inset in image second from left) mark FVF^low epiblast progenitors in the epiblasts, whereas the red asterisk (rightmost image inset in white dashed box) and red dashed line (inset in image second from left) indicate FVF^high transitory progenitors that express E-cadherin and N-cadherin. The primitive streak region is indicated by N-cadherin expression (blue dashed line in rightmost image inset in dashed yellow box). a′–c′, Depiction of Foxa2 (a′), T and Snail1 (b′) and E-cadherin and N-cadherin expression (c′) in gastrulating embryos based on the embryos in a–c. d, Transverse section through the epiblast of a mid-streak-stage wild-type embryo immunostained for Foxa2, E-cadherin and N-cadherin. e,f, Western blot analysis (e) and quantification (f) of E-cadherin from FACS-sorted FVF^neg, FVF^low and FVF^high cells of 122 and 36 FVF embryos (n = 2). The asterisk marks unspecific bands. All shown confocal images are single z planes of a z stack. The images in a–d are representative of eight, three, three and three embryos, respectively. All samples were derived from biologically independent experiments. The data are presented as mean values. Scale bars, 50 µm (insets, 10 µm).

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