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. 2021 Mar 16;156(1):35–46. doi: 10.1007/s00418-021-01980-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Modifications of PGP9.5 expression at 1 and 7 days of culture in ASCs undergoing different treatments. In panel a (1 day) and b (7 days), PGP9.5 immunoreactivity is shown in different groups of ASCs: ASC cultures in the basal medium (ASC); ASC cultures in OEC-CM (ASC/OEC-CM) or SC-CM (ASC/SC-CM); ASC cultures where ghrelin was added to the basal medium (ASC + Ghre), to OEC-CM (ASC/OEC-CM + Ghre) or to SC-CM (ASC/SC-CM + Ghre). In each flow cytometry plot, data obtained from each culture condition, with or without ghrelin, are merged. Histograms show cell frequency distribution of each population, according to FITC fluorescence intensity expressed in logarithmic scale. Histogram overlays are displayed as %Max, scaling each curve to the respective modal values = 100%. Scale bar: 50 μm