Figure 4: Genome-wide profiling of EGFR-regulated TAZ gene targets in GBM neurospheres.

A: Outline of the genome-wide analysis.

B: Heatmap clustering of 6 RNA-Seq samples from 3 conditions. Gene expression was calculated by RPKM.

C: Volcano plot showing DE genes (red and blue dots: genes with FDR ≤ 0.05 and log₂(fold change) ≥ 0.8 or ≤ −0.8, respectively).

D: ChIP-Seq reproducibility was analyzed by scatter plot comparing peak intensities for two ChIP-Seq replicates.

E: TAZ binding peaks show enrichment around TSSs, compared to randomly selected genomic regions.

F: Overlapping between TAZ peaks and histone marks as marked.

G: TAZ binding motif was identified by de novo motif discovery and compared to TEAD3 and TEAD1 motifs.