KEY RESOURCES
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| rabbit anti-TAZ | Cell Signaling Technology | # 4883 |
| mouse anti-Actin | Sigma-Aldrich | # A1978 |
| rabbit anti-TBP | Cell Signaling Technology | # 40059 |
| rabbit anti-YAP | Cell Signaling Technology | # 14074 |
| rabbit anti-phospho-LATS1 | Cell Signaling Technology | # 9157 |
| rabbit anti-LATS1 | Cell Signaling Technology | # 9153 |
| rabbit anti-phospho-STAT3 | Cell Signaling Technology | # 9145 |
| mouse anti-STAT3 | Cell Signaling Technology | # 9139 |
| rabbit anti-phospho-ERK1/2 | Cell Signaling Technology | # 4370 |
| mouse anti-ERK1/2 | Cell Signaling Technology | # 4696 |
| rabbit anti-phospho-AKT | Cell Signaling Technology | # 4060 |
| mouse anti-AKT | Cell Signaling Technology | # 2920 |
| rabbit anti-EGFR | Cell Signaling Technology | # 4267 |
| rabbit anti-phospho-EGFR | Cell Signaling Technology | # 2231 |
| rabbit anti-PDGFRβ | Cell Signaling Technology | # 3169 |
| rabbit anti-phospho-PDGFRβ | Cell Signaling Technology | # 3166 |
| Rabbit anti-phospho-FGFR1–4 | R&D | # AF3285-SP |
| Rabbit anti-FGFR1 | Cell Signaling Technology | # 9740 |
| rabbit anti-phospho-MET | Cell Signaling Technology | # 3077 |
| rabbit anti-MET | Cell Signaling Technology | # 8198 |
| rabbit anti-JUN | Cell Signaling Technology | # 9165 |
| rabbit anti-CD274 | Cell Signaling Technology | # 13684 |
| rabbit anti-CXCR4 | Thermo Fisher | # 35–8800 |
| rabbit anti-HIF1A | Cell Signaling Technology | # 36169 |
| rabbit anti-JAK2 | Cell Signaling Technology | # 3230 |
| rabbit anti-PDGFRA | Cell Signaling Technology | # 3174 |
| rabbit anti-TEAD1 | Cell Signaling Technology | #12292 |
| mouse anti-FLAG HRP | Sigma-Aldrich | # A8592 |
| Rabbit anti-Ki67 | Cell Signaling Technology | #9129 |
| mouse anti-human Nuclei | Millipore | #MAB1281 |
| rabbit anti-STAT3 (ChIP) | Cell Signaling Technology | # 12640 |
| rabbit IgG (ChIP) | Cell Signaling Technology | # 2729 |
| Bacterial and Virus Strains | ||
| STBL3 Competent E. coli | Thermo Fisher | C737303 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Osimertinib (0.5μM for screening) | Medkoo | # 206042 |
| AEE788 (0.05μM for screening) | Cayman | # 18416 |
| PF299804 (0.1μM for screening) | Cayman | # 9001879 |
| Lapatinib (0.2μM for screening) | Medkoo | # 100490A |
| BPI-2009 (10μM for screening) | Medkoo | # 205897 |
| Lazertinib (0.1μM for screening) | Medkoo | # 206860 |
| Lidocaine hydrochloride (1mM for screening) | Selleckchem | # S4667 |
| Erlotinib (10μM for screening) | Cell Signaling Technology | # 5083 |
| Gefitinib (10μM for screening) | Cell Signaling Technology | # 4765 |
| JNJ-42756493 (0.05μM for screening) | Cayman | # 21813 |
| Sunitinib (1μM for screening) | Cayman | # 13159 |
| Sorafenib (1μM for screening) | Cayman | # 10009644 |
| 5,15-DPP | Santa Cruz | # sc-204305 |
| Stattic | Santa Cruz | # sc-202818 |
| PD98059 | Cell Signaling Technology | # 9900 |
| FR180204 | Sigma-Aldrich | # SML0320 |
| Akti-1/2 | Tocris | # 5773 |
| Critical Commercial Assays | ||
| Trans-Lentiviral Packaging System | Thermo Fisher | TLP4614 |
| PDGFβ ELISA Kit | R&D Systems | DBB00 |
| EGF ELISA Kit | R&D Systems | DEG00 |
| alamarBlue Cell Viability Reagent | Thermo Fisher | DAL1025 |
| Illumina TrueSeq RNA Sample Preparation kit v2 | Illumina | RS-122–2001 |
| ChIP-Seq DNA Sample Prep kit | Illumina | IP-202–1012 |
| MAGnify ChIP system | Thermo Fisher | 492024 |
| RNeasy kit | Qiagen | 74104 |
| Deposited Data | ||
| ChIP-seq datasets | This paper | GEO GSE151994 |
| RNA-seq datasets | This paper | GEO GSE151995 |
| Experimental Models: Cell Lines | ||
| GBM1B, GBM1A | Dr. Vescovi (Galli et al., 2004) | |
| M1123 (1123) | Dr. Nakano (Mao et al., 2013) | |
| U87MG | ATCC | HTB-14 |
| A172 | ATCC | CRL 1620 |
| 293FT | Thermo Fisher | R70007 |
| Experimental Models: Organisms/Strains | ||
| NOD/SCID mice (female, 6–8 weeks) | Jackson Laboratory | 001303 |
| Oligonucleotides | ||
| Primers for qRT-PCR, see below | This paper | |
| Primers for ChIP-PCR, see below | This paper | |
| Recombinant DNA | ||
| pLEX-MCS lentiviral vector | Thermo Scientific | OHS4735 |
| GCGATGAATCAGCCTCTGAAT (shTAZ1 lentiviral shRNA vector) | Sigma-Aldrich | TRCN0000019469 |
| CCAGGAACAAACGTTGACTTA (shTAZ2 lentiviral shRNA vector) | Sigma-Aldrich | TRCN0000019470 |
| pLKO.1-puro TurboGFP™ Control shRNA Lentiviral Vector | Sigma-Aldrich | SHC004 |
| EGFRvIII ORF | Dr. Alonzo Ross | Addgene #20737 |
| TAZ ORF | Dr. Kunliang Guan | Addgene #32839 |
| Software and Algorithms | ||
| Bowtie2 | Langmead and Salzberg, 2012 | |
| MACS2 | Zhang et al., 2008 | |
| BEDTools | Lawrence et al., 2013 | |
| CisGenome | Ji et al., 2008 | |
| HOMER | Heinz et al., 2010 | |
| Primers | ||
| qRT-PCR | ||
| Gene | Primer 5’ | Primer 3’ |
| TAZ | GATCCTGCCGGAGTCTTTCTT | CACGTCGTAGGACTGCTGG |
| CTGF | CCAATGACAACGCCTCCTG | TGGTGCAGCCAGAAAGCTC |
| MYC | CCTGGTGCTCCATGAGGAGAC | CAGACTCTGACCTTTTGCCAGG |
| 18s | ACAGGATTGACAGATTGATAGCTC | CAAATCGCTCCACCAACTAAGAA |
| EGFR | AACACCCTGGTCTGGAAGTACG | TCGTTGGACAGCCTTCAAGACC |
| PDGFB | GAGATGCTGAGTGACCACTCGA | GTCATGTTCAGGTCCAACTCGG |
| EGF | TGCGATGCCAAGCAGTCTGTGA | GCATAGCCCAATCTGAGAACCAC |
| PDGFRA | GACTTTCGCCAAAGTGGAGGAG | AGCCACCGTGAGTTCAGAACGC |
| PDGFA | CAGCGACTCCTGGAGATAGACT | CGATGCTTCTCTTCCTCCGAATG |
| VEGFA | TTGCCTTGCTGCTCTACCTCCA | GATGGCAGTAGCTGCGCTGATA |
| FGF2 | AGCGGCTGTACTGCAAAAACGG | CCTTTGATAGACACAACTCCTCTC |
| FGF1 | ATGGCACAGTGGATGGGACAAG | TAAAAGCCCGTCGGTGTCCATG |
| FGFR4 | AACACCGTCAAGTTCCGCTGTC | CATCACGAGACTCCAGTGCTGA |
| ERBB3 | CTATGAGGCGATACTTGGAACGG | GCACAGTTCCAAAGACACCCGA |
| JUN | CCTTGAAAGCTCAGAACTCGGAG | TGCTGCGTTAGCATGAGTTGGC |
| HIF1A | TATGAGCCAGAAGAACTTTTAGGC | CACCTCTTTTGGCAAGCATCCTG |
| JAK2 | CCAGATGGAAACTGTTCGCTCAG | GAGGTTGGTACATCAGAAACACC |
| CXCR4 | CTCCTCTTTGTCATCACGCTTCC | GGATGAGGACACTGCTGTAGAG |
| CD274 | TGCCGACTACAAGCGAATTACTG | CTGCTTGTCCAGATGACTTCGG |
| TEAD1 | CCTGGCTATCTATCCACCATGTG | TTCTGGTCCTCGTCTTGCCTGT |
| ChIP-PCR | ||
| Gene | Primer 5’ | Primer 3’ |
| EGFR | CGTCCTTTCCTGTTTCCTTG | GGGGAAAGTGAGGGAAGAAA |
| PDGFB | ACTGAATGGGCAAAAACTGG | TGCAAACAGGGACAATTTGA |
| EGF | TATTGGCTGTGGGTTTGTCA | ACCATGATCAAGTGGGCTTC |
| PDGFRA | CAGGTGGATTGCTTGAGGTC | CTTGAGTGCAATGGCATGAT |
| PDGFA | CTGAGCCCAGAGTCAGGTTC | GGAGTAGCGACTGCATGTGA |
| VEGFA | CCAACTTCTGGGCTGTTCTC | CCCCTCTCCTCTTCCTTCTC |
| FGF2 | ATGATGACAATGGCGGTTTT | GGATCCCAAGGCAGAAGAAT |
| FGF1 | TGCCTTTTGCCTTTGACTCT | ATGGAGGAGCTGAGCTTTGA |
| FGFR4 | CCCAGAGCCAGTTCACAAAC | CGAGTAAGGAGCTTGGCAGT |
| ERBB3 | GTTGGGGGTAAGGTCACAGA | GAATAGAAAGGCGGGAAAGG |
| HIF1A | CATCACTGGCCATCAGAGAA | TGGCTGGGTCAAATGGTATT |
| JAK2 | AAATGGTGCTGGGAAAACTG | GCCATTGCTTTTGGTGTTTT |
| CXCR4 | TTCTGGGCTTCAAGCAACTT | AAACGCGCCAAGTGATAAAC |
| CD274 | CAGCACCTGTTGTTTCCTGA | GGGCGAAGGATATGAACAGA |
| TEAD1 | GTCCTGGCGTTCATTTCATT | AGTCTGCAAAGTGCTTGGTG |
| Negative Control NC1 | GTCTGTACTCCCAGCTACTC | GACAGAGCAAGACTCCATC |
| Negative Control NC2 | GACTGGTTCATTCACTGCTC | CACTTCCCTACCCGAAAC |
| STAT3 ChIP-PCR | TCCTCTTCCTCCTCCTCCTC | AAAGGAATGCAGATGCAGGT |