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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Dev Biol. 2021 May 20;477:164–176. doi: 10.1016/j.ydbio.2021.05.006

Figure 2. Mouse LZTFL1 protein and mRNA tissue distribution.

Figure 2.

A. Analysis of tissue distribution of mouse LZTFL1 protein by Western blot. 50ug of total protein was loaded and the protein was examined by less sensitive Pico system. The anti-LZTFL1 antibody concentration: 1:2000. LZTFL1 protein is abundant only in the testis.

B. Analysis of relative Lztfl1 mRNA expression in adult mouse tissues by RT-PCR (a) and qPCR (b). Lztfl1 was detected in all tissues, but most abundant in testis. Lztfl1 mRNA expression levels were normalized by Gapdh.

C. LZTFL1 expression levels during the first wave of spermatogenesis. LZTFL1 was detectable from postnatal day 16; its expression was up-regulated at day 28, and further increased on days 30 and 35. Thus, the protein level was elevated during spermiogenesis, the final phase of spermatogenesis.