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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Dev Biol. 2021 May 20;477:164–176. doi: 10.1016/j.ydbio.2021.05.006

Figure 3. Localization of LZTFL1 in normal male germ cells.

Figure 3.

A. Localization of LZTFL1 in isolated germ cells was examined by immunofluorescence staining. LZTFL1 signal was observed in the cytoplasm of spermatocytes (panel a, arrows) and round spermatids (panels a and b, dotted arrows). Some signal appears near the manchette of elongating spermatids (panel b, arrow head), and is also localized in the developing sperm tails (panel c, star). No LZTFL1 signal was observed in the tails of the developed sperm (panel d). Images were taken with a confocal microscope.

B. LZTFL1 is not expressed in the epididymal sperm. Epididymal sperm were collected from cauda epididymis, and Western blot was conducted. LZTFL1 protein was detected in the testis, but not in sperm.