A. Representative Western blot result showing that LZTFL1 protein was missing in the testis of global Lztfl1 KO mice.
B, C. Percentage of motile sperm and sperm motility was reduced in the Lztfl1 KO mice (n=10). Statistically significant differences: *p < 0.05.
D. Quantification of the percentage of different types of abnormal sperm in the control (wild type and heterozygote) and Lztfl1 KO mice (n=10). Statistically significant differences: *p < 0.05.
E. Representative images of epididymal sperm under a light microscopy from the control (heterozygote) (a) and Lztfl1 KO mice (b-f). Sperm from the KO mice showed uneven thickness tail (b, c), misshaped head (b-d), short tail (d-f), and swollen tail tips (d, f).